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chip-seq library preparation and sequencing  (Azenta)

 
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    Azenta chip-seq library preparation and sequencing
    Chip Seq Library Preparation And Sequencing, supplied by Azenta, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chip-seq library preparation and sequencing/product/Azenta
    Average 90 stars, based on 1 article reviews
    chip-seq library preparation and sequencing - by Bioz Stars, 2026-05
    90/100 stars

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    PHF20 and PHF20L1 bind to the promoters of highly expressed genes. A , Venn diagram indicating the numbers and the overlap of 3xHA PHF20 and 3xHA <t>PHF20L1</t> <t>ChIP-Seq</t> peaks. B , pie chart of the genomic distribution of 3xHA PHF20-binding ( left ) and 3xHA PHF20L1-binding sites. C , average normalized input and HA ChIP-Seq profiles of 3xHA PHF20 ( left ) and 3xHA PHF200L1 ( right ) across transcription start sites (TSSs). D , Venn diagram indicating the numbers and overlap of 3xHA PHF20, 3xHA PHF20L1, and KANSL3 ChIP-Seq peaks. E , boxplot showing the expression of genes that do not have PHF20 or PHF20L1 binding to their promoters (nonbound), genes that have both PHF20 and PHF20L1 binding to their promoters (dual targets), or genes that have only PHF20 binding to their promoters (PHF20_only targets) in U2OS cells. ∗∗∗∗ p (by two-sided t test) <0.0001. ChIP-Seq, chromatin immunoprecipitation sequencing; HA, hemagglutinin; PHF20, plant homeodomain finger protein 20; PHF20L1, PHF20-like protein 1.
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    PHF20 and PHF20L1 bind to the promoters of highly expressed genes. A , Venn diagram indicating the numbers and the overlap of 3xHA PHF20 and 3xHA PHF20L1 ChIP-Seq peaks. B , pie chart of the genomic distribution of 3xHA PHF20-binding ( left ) and 3xHA PHF20L1-binding sites. C , average normalized input and HA ChIP-Seq profiles of 3xHA PHF20 ( left ) and 3xHA PHF200L1 ( right ) across transcription start sites (TSSs). D , Venn diagram indicating the numbers and overlap of 3xHA PHF20, 3xHA PHF20L1, and KANSL3 ChIP-Seq peaks. E , boxplot showing the expression of genes that do not have PHF20 or PHF20L1 binding to their promoters (nonbound), genes that have both PHF20 and PHF20L1 binding to their promoters (dual targets), or genes that have only PHF20 binding to their promoters (PHF20_only targets) in U2OS cells. ∗∗∗∗ p (by two-sided t test) <0.0001. ChIP-Seq, chromatin immunoprecipitation sequencing; HA, hemagglutinin; PHF20, plant homeodomain finger protein 20; PHF20L1, PHF20-like protein 1.

    Journal: The Journal of Biological Chemistry

    Article Title: Methyl-lysine readers PHF20 and PHF20L1 define two distinct gene expression–regulating NSL complexes

    doi: 10.1016/j.jbc.2022.101588

    Figure Lengend Snippet: PHF20 and PHF20L1 bind to the promoters of highly expressed genes. A , Venn diagram indicating the numbers and the overlap of 3xHA PHF20 and 3xHA PHF20L1 ChIP-Seq peaks. B , pie chart of the genomic distribution of 3xHA PHF20-binding ( left ) and 3xHA PHF20L1-binding sites. C , average normalized input and HA ChIP-Seq profiles of 3xHA PHF20 ( left ) and 3xHA PHF200L1 ( right ) across transcription start sites (TSSs). D , Venn diagram indicating the numbers and overlap of 3xHA PHF20, 3xHA PHF20L1, and KANSL3 ChIP-Seq peaks. E , boxplot showing the expression of genes that do not have PHF20 or PHF20L1 binding to their promoters (nonbound), genes that have both PHF20 and PHF20L1 binding to their promoters (dual targets), or genes that have only PHF20 binding to their promoters (PHF20_only targets) in U2OS cells. ∗∗∗∗ p (by two-sided t test) <0.0001. ChIP-Seq, chromatin immunoprecipitation sequencing; HA, hemagglutinin; PHF20, plant homeodomain finger protein 20; PHF20L1, PHF20-like protein 1.

    Article Snippet: HA-ChIPs and library preparation for sequencing PHF20-bound and PHF20L-bound DNA (ChIP-Seq) were performed at MD Anderson Epigenomics Profiling Core as described earlier ( ).

    Techniques: ChIP-sequencing, Binding Assay, Expressing